Technical Notes & Presentations

Training Documentation

PC Based MicroCT50 SOP (with video timestamps, use with video below)

SCANCO microCT50 Scanning/Analysis Tutorial video

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Instructions: VMS MicroCT scanning and analysis (PDF)

Note: Scanco training documents are available on request for local Core users. Please contact Michael Brodt for information

Step by Step Training Videos for VMS Systems

• Step 1: Starting a new experiment on the microCT50
• Step 2: Loading the microCT50
• Step 3: Starting a set of scans on the microCT50
• Step 4: Choosing evaluation script thresholds
• Step 5: Cortical analysis
• Step 6: Trabecular analysis
• Step 7: Exporting data to Excel
• Step 8: Generating 3D images
• Step 9: Retrieving contour data after clearing
• Steps 1-9 (Playlist)

Background Documentation

MicroCT for scanning and analysis of mouse bones (Skeletal Development and Repair pp 169-198)

Forms

Standard arrangement uCT50 scanning parameters

Generic uCT50 scanning parameters

Slice Analysis Table

• Understanding uCT outcomes
• Understanding 3pt bending outcomes
• Understanding RPI outcome

• Isoflurane system instructions
• Guidelines for assessment of bone microstructure
• Guidelines for evaluating long bone phenotypes
• Understanding muscle physiology
• Long bone uCT scanning followed by histology

Study design sample size calculation for uCT

Radiation dose and its effects

• Brouwers (JOR 2007)
• Klinck (Med Eng Phy 2008)
• Laperre (Bone 2011)

• Bone imaging and mechanical testing (2014)
• Using bone imaging and mechanical testing (2016)
• Using bone imaging and mechanical testing for skeletal phenotyping (seminar recording – 2018)
• Using bone imaging and mechanical testing for skeletal phenotyping(PDF – 2018)
• Methods for quantifying skeletal muscle pathology (seminar recording – 2018)
• Structure and strength assessments (2020)