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Washington University School of Medicine in St. Louis

Musculoskeletal Research Center

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Technical Notes & Presentations

microCT50 Training

Training Documentation

Instructions: MicroCT scanning and analysis (PDF)

Note: Scanco training documents are available on request for local Core users. Please contact Michael Brodt for information

Step by Step Training Videos

  • Step 1: Starting a new experiment on the microCT50
  • Step 2: Loading the microCT50
  • Step 3: Starting a set of scans on the microCT50
  • Step 4: Choosing evaluation script thresholds
  • Step 5: Cortical analysis
  • Step 6: Trabecular analysis
  • Step 7: Exporting data to Excel
  • Step 8: Generating 3D images
  • Step 9: Retrieving contour data after clearing
  • Steps 1-9 (Playlist)

Background Documentation

MicroCT for scanning and analysis of mouse bones (Skeletal Development and Repair pp 169-198)

Forms

Standard arrangement uCT50 scanning parameters

Generic uCT50 scanning parameters

Understanding Core B Outcomes

  • Understanding uCT outcomes
  • Understanding 3pt bending outcomes
  • Understanding RPI outcome

Additional Content and Instructions

  • Isoflurane system instructions
  • Guidelines for assessment of bone microstructure
  • Guidelines for evaluating long bone phenotypes
  • Understanding muscle physiology
  • Long bone uCT scanning followed by histology

Practical Scanning Issues

Study design sample size calculation for uCT

Radiation dose and its effects

  • Brouwers (JOR 2007)
  • Klinck (Med Eng Phy 2008)
  • Laperre (Bone 2011)

Lectures

  • Bone imaging and mechanical testing (2014)
  • Using bone imaging and mechanical testing (2016)
  • Using bone imaging and mechanical testing for skeletal phenotyping (seminar recording – 2018)
  • Using bone imaging and mechanical testing for skeletal phenotyping(PDF – 2018)
  • Methods for quantifying skeletal muscle pathology (seminar recording – 2018)
  • Structure and strength assessments (2020)
  • Cores
    • Core A – Administrative Core
    • Core B – Structure & Strength
      • People
      • Services
      • Core B Consultation Request
      • MRC/WUCCI Partnership for 3D X-Ray Imaging
      • Technical Notes & Presentations
      • Equipment
    • Core C – Histology & Morphometry
    • Core D – Animal Models of Bone and Joint Injury & Disease
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Musculoskeletal Research Center

425 S. Euclid Ave.
11th Floor
St. Louis, MO 63110

Citing the grant in publications:

“Washington University Musculoskeletal Research Center (NIH P30 AR074992).”

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