Technical Notes & Presentations

Training Documentation

Instructions: MicroCT Scanning and Analysis (PDF)

Note: Scanco training documents are available on request for local Core users. Please contact Michael Brodt for information

Step by Step Training Videos

• Step 1: Starting a new experiment on the microCT50
• Step 2: Loading the microCT50
• Step 3: Starting a set of scans on the microCT50
• Step 4: Choosing evaluation script thresholds
• Step 5: Cortical Analysis
• Step 6: Trabecular Analysis
• Step 7: Exporting data to Excel
• Step 8: Generating 3D images
• Step 9: Retrieving Contour Data After Clearing
• Steps 1-9 (Playlist)

Background Documentation

MicroCT for Scanning and Analysis of Mouse Bones (Skeletal Development and Repair pp 169-198)

Forms

Standard Arrangement uCT50 Scanning Parameters

Generic uCT50 Scanning Parameters

Additional Content & Instructions

Long Bone uCT Scanning Followed by Histology

• Understanding uCT Outcomes
• Understanding 3pt Bending Outcomes
• Understanding RPI Outcomes
• Guidelines for evaluating long bone phenotypes
• Understanding muscle physiology

• Isoflurane system

• Flow Chart
• Study design sample size calculation for uCT
• Radiation dose and its effects

Supporting documentation:

• Boyd (Bone 2006)
• Brouwers (CTI 2008)
  • Brouwers (JOR 2007)
  • Buie (Bone 2007)
  • Buie (JBMR 2008)
  • Cartmell (JBMRA 2004)
  • Glatt (JBMR 2007)
  • Klinck (CTI 2008)
  • Klinck (Med Eng Phy 2008)
  • Laperre (Bone 2011)
  • Willie (Bone 2013)

• Bone Imaging and Mechanical Testing (2014)
• Using Bone Imaging and Mechanical Testing (2016)
• Using Bone Imaging and Mechanical Testing for Skeletal Phenotyping (Seminar recording – 2018)
• Using Bone IMaging and Mechanical Testing for Skeletal Phenotyping(PDF – 2018)
• Methods for Quantifying Skeletal Muscle Pathology (Seminar recording – 2018)
• Structure and Strength Assessments (2020)

• Boyd Auto Contour
• Hierarchical Microimaging of Bone Structure
• MicroCT Guidelines