Paraffin, frozen, and resin histology. Special stains and advanced techniques available as detailed below. Have a question or need help with a new project? Contact us for a complimentary consultation. All requests for Core services and equipment scheduling are available through iLabs.
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Fee Schedule *Members of the MRC Research Base receive 33% discount on all fees. For users outside of Washington University, please contact us for fees.
Surcharges & Discounts
Blocks (per block) Paraffin Processing $10.00 Paraffin Processing + decal by core $24.00 Plastic embedding $44.00
Slides (per slide) Paraffin section $2.15 Plastic section $12.25 micro/orientation fee 50% extra/section H & E stain $7.85 Special stains (view list) $12.15 Deplast.+coverslip $4.95
Frozen Sections Frozen section $4.72 Non-decal section of bone $15.72 Frozen section on film $15.72 Coverslip w/o prior stain $1.57
Hourly Fees Microtome Use $11.00/hour Technician Time $44.00/hour Confocal $15.00/hour BioQuant $10.00/hour
Supplies large slide box (100) $6.60 small slide box (25) $4.13 Microtome blades (ea) $1.80 Slides (box of 100) $24.75 EDTA Decalcification Solution 1 Liter free if the samples will be submitted to the core for processing
Surcharges & Discounts Priority service feerequires prior PI approval 50% Core Member discount 33%
Estimated Time Schedule for Services Paraffin processing/sectioning/staining <= 20 blocks, no special requests 1-2 weeks <= 20 blocks, microscope for orientation or leveling through (50+ slides per block) 2-4 weeks > 20 blocks, no special requests 2-4 weeks > 20 blocks, microscope for orientation or leveling through (50+ slides per block) 4-8 weeks
MMA (plastic) processing/sectioning/staining <= 20 blocks 4-8 weeks > 20 blocks 8-12 weeks
Cryostat sectioning Routine 2-4 weeks For LCM Can be scheduled Priority (Rush) work Less than 5 work days, needs PI approval, with 50% surcharge
Special Stains
(If you do not see your preferred stain, let us know. We accept special requests.)
Alcian Blue
Alcian Blue Hematoxylin Orange G
Alizarin Red
Bone Canaliculi Silver Stain
Geimsa
Goldner’s Trichrome
Gomori’s & Masson Trichrome
Gram Stain
Oil Red O
Pentachrome
Periodic acid-Schiff (PAS)
Perl’s Iron
Picosirius Red/Alcian Blue
PTAH (Phosphotungstic Acid Hematoxylin)
Reticulin
Safranin O (may NOT decalcify with EDTA as this leads to removal of the proteoglycans; must use Formic acid/Immunocal/CalEx)
Toluidine Blue
TRAP (Tartrate resistant acid phosphatase; must use neutral EDTA decalcification)
Verhoff van-Giesen (VVG)
Von Kossa
Weigert’s Resorcin Fushsin
Advanced Processing Methods
RNA Scope
CODEX Multiplexed Immunofluorescence
Spatial Transcriptomatic RNA analysis