Paraffin, frozen, and resin histology. Special stains and advanced techniques available as detailed below. Have a question or need help with a new project?
Contact us for a complimentary consultation. All requests for Core services and equipment scheduling are available through iLabs.
*Members of the MRC Research Base receive 33% discount on all fees.For users outside of Washington University, please contact us for fees. Surcharges & Discounts
Blocks (per block) Paraffin Processing $10.00 Paraffin Processing + decal by core $24.00 Plastic embedding $44.00
Slides (per slide) Paraffin section $2.15 Plastic section $12.25 micro/orientation fee 50% extra/section H & E stain $7.85 Special stains (view list) $12.15 Deplast.+coverslip $4.95
Frozen Sections Frozen section $4.72 Non-decal section of bone $15.72 Frozen section on film $15.72 Coverslip w/o prior stain $1.57
Hourly Fees Microtome Use $11.00/hour Technician Time $44.00/hour Confocal $15.00/hour BioQuant $10.00/hour
Supplies large slide box (100) $6.60 small slide box (25) $4.13 Microtome blades (ea) $1.80 Slides (box of 100) $24.75
Surcharges & Discounts Priority service fee requires prior PI approval 30% Core Member discount 33%
Estimated Time Schedule for Services
Paraffin processing/sectioning/staining <= 20 blocks, no special requests 1-2 weeks <= 20 blocks, microscope for orientation or leveling through (50+ slides per block) 2-4 weeks > 20 blocks, no special requests 2-4 weeks > 20 blocks, microscope for orientation or leveling through (50+ slides per block) 4-8 weeks
MMA (plastic) processing/sectioning/staining <= 20 blocks 4-8 weeks > 20 blocks 8-12 weeks
Cryostat sectioning Routine 2-4 weeks For LCM Can be scheduled Priority (Rush) work Not available for MMA processing Less than 5 work days, needs PI approval, with 30% surcharge
(If you do not see your preferred stain, let us know. We accept special requests.)
Alcian Blue Alcian Blue Hematoxylin Orange G Alizarin Red Bone Canaliculi Silver Stain Geimsa Goldner’s Trichrome Gomori’s & Masson Trichrome Gram Stain Oil Red O Pentachrome Periodic acid-Schiff (PAS) Perl’s Iron Picosirius Red/Alcian Blue PTAH (Phosphotungstic Acid Hematoxylin) Reticulin Safranin O Toluidine Blue TRAP (Tartrate resistant acid phosphatase) Verhoff van-Giesen (VVG) Von Kossa Weigert’s Resorcin Fushsin Advanced Processing Methods
RNA Scope CODEX Multiplexed Immunofluorescence Spatial Transcriptomatic RNA analysis