Technical Notes & Presentations

Protocols:

• Fixation Protocols (word doc)
• Decalcification Protocol (word doc) (EDTA requests must be made a week in advance)
• Mouse Calcein Alizarin Protocol (pdf)
• Immunostaining of Skeletal Tissues (overview article, pdf)
• Immunostaining of Frozen Sections (detailed step-by-step protocol, link)
• RNAscope and GeoMx in Skeletal Tissues (detailed step-by-step protocol, pdf)

Presentations and Technical Notes:

• Bone Histomorphometry Guidelines (JBMR article, pdf)
• Bone Histomorphometry (Video Lecture, 2020)
• Bone Histomorphometry 101 (pdf)
• Long Bone uCT Scanning followed by Histology

Protocols:

• BioQuant Protocol (pdf)
• Bioquant Calibration Troubleshooting (pdf)

Software:

• Leica LAS X offline software (free download)